Vectors {Bacterial Artificial Chromosome} (BAC) derived from F-factor plasmids can clone 100,000-base to 300,000-base DNA fragments in Escherichia coli.
Cloning-vector plasmids {cosmid} can contain lambda-phage cos gene, infect E. coli, and clone DNA fragments up to 45,000 bases between phage-end cos sites.
Bacteria can have 5000-base circular DNAs {plasmid}| that can insert up to 1000 bases. Cells can have 10 to 200 independently replicating plasmids {relaxed-control plasmid}. Plasmids {stringent-control plasmid} can replicate together with bacterial chromosomes. Artificial plasmids can be cloning vectors.
4-Genetics-Recombinant DNA-Cloning-Vector
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Date Modified: 2022.0225